In Vitro Production of Ergothioneine Isotopologues

Ergothioneine is an emerging component of the redox homeostasis system in human cells and microbial pathogens, such as Mycobacterium tuberculosis Burkholderia pseudomallei. The synthesis stable isotope-labeled ergothioneine derivatives may provide important tools for deciphering distribution, function, metabolism this compound vivo. We describe a general protocol production isotopologues with programmable 2H, 15N, 13C, 34S, 33S isotope labeling patterns. This enzyme-based approach makes efficient use commercial reagents also directly applicable to radio-isotopologues.